Effects of Hydroxamic Acids Isolated from Gramineae on Adenosine 5′-triphosphate Synthesis in Chloroplasts

Antidipsogenic effects of central adenosine-5'-triphosphate.

Besides other physiological functions, adenosine-5'-triphosphate (ATP) is also a neurotransmitter that acts on purinergic receptors. In spite of the presence of purinergic receptors in forebrain areas involved with fluid-electrolyte balance, the effect of ATP on water intake has not been investigated. Therefore, we studied the effects of intracerebroventricular (icv) injections of ATP (100, 200...

Studies on the Hydrolysis of Adenosine Triphosphate by Spinach Chloroplasts.

During studies on photophosphorylation in cell-free preparations of the blue-green alga, Anabaena variabilis, a photohydrolysis of ATP was found to be induced by high concentrations of cysteine or GSH, while photophosphorylation became inhibited (1, 2). A similar photohydrolysis was also reported with chloroplasts from spinach leaves, but still higher sulfhydryl concentrations were required to ...

Electrophysiologic effects of adenosine-5'-triphosphate on atrioventricular reentrant tachycardia.

Adenosine 5'-triphosphate sulphurylase from Saccharomyces cerevisiae.

1. ATP sulphurylase from Saccharomyces cerevisiae was purified 140-fold by using heat treatment, DEAE-cellulose chromatography and Sepharose 6B gel filtration. 2. The enzyme was stable at -15 degrees C, optimum reaction velocity was between pH7.0 and 9.0, and the activation energy was 62kJ/mol (14.7kcal/mol). 3. The substrate was shown to be the MgATP(2-) complex, free ATP being inhibitory. 4. ...

Inhibitory action of extracellular adenosine 5'-triphosphate on parietal cells isolated from rabbit gastric mucosa.

The effects of the purine nucleotides, adenosine 5'-triphosphate (ATP) and their analogs 2-methylthio ATP and beta, gamma-methylene ATP, as well as those of the pyrimidine nucleotide, uridine 5'-triphosphate (UTP), on acid production in isolated rabbit gastric parietal cells prepared by enzymatic dispersion and enriched by counterflow elutriation were studied. The (14C)-aminopyrine (AP) accumul...